产品基本信息

• 产品货号 BD-PN2071
• 产品名称 N2DL2 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 ULBP2 N2DL2 RAET1H UNQ463/PRO791
• 蛋白名称 NKG2D ligand 2 (N2DL-2) (NKG2DL2) (ALCAN-alpha) (Retinoic acid early transcript 1H) (UL16-binding protein 2)
• Clonality Polyclonal
• 推荐应用 WB,ELISA
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
• Human Gene ID 80328
• Human Swissprot Link https://www.uniprot.org/uniprotkb/Q9BZM5/entry
• 免疫原 Synthesized peptide derived from part region of human protein
• 特异性 N2DL2 Polyclonal Antibody detects endogenous levels of protein.
• 稀释度 WB 1:500-2000, ELISA 1:5000-20000
• 预测分子量 27kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015],
• 组织表达 Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues.
• 细胞定位 Cell membrane ; Lipid-anchor, GPI-anchor . Endoplasmic reticulum . Secreted . In CMV-infected fibroblasts, detected in the endoplasmic reticulum/cis-Golgi. .
• 信号通路 Natural killer cell mediated cytotoxicity;
• 功能 function:Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.,miscellaneous:The ULBPs are unusual members of the extended MHC class I superfamily, because they do not contain the alpha 3 domain and they lack a transmembrane domain. They are unlikely to present peptides.,similarity:Belongs to the MHC class I family.,subunit:Interacts with the CMV glycoprotein UL16. Does not bind to beta2-microglobulin.,tissue specificity:Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night

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