新品上线| mIHC多重荧光免疫组化试剂盒

mIHC（多重荧光免疫组化）是在IHC基础上，基于酪酰胺信号放大(TSA，Tyramide signal amplification)技术，实现的同一张切片的多指标荧光检测。荧光素标记酪酰胺（Tyramide）在二抗携带的HRP酶促作用下，生成活化荧光底物与靶标蛋白的酪氨酸共价结合，从而使靶标蛋白标记上特定的荧光；随后通过加热剥离洗去非共价结合的抗体，再重复操作换下一个指标的一抗，搭配另一种荧光素标记的Tyramide进行染色，如此往复可在同一张切片上实现多指标同时分析。


 

货号	mIHC试剂盒套装	规格
BDAA0200	鼠兔三标四色荧光检测试剂盒(pH9.0)	1ml/2ml/5ml
BDAA0201	鼠兔双标三色荧光检测试剂盒(pH9.0)	1ml/2ml/5ml
BDAA0202	鼠兔四标五色荧光检测试剂盒(pH9.0)	1ml/2ml/5ml
BDAA0203	鼠兔五标六色荧光检测试剂盒(pH9.0)	1ml/2ml/5ml
BDAA0204	鼠兔六标七色荧光检测试剂盒(pH9.0)	1ml/2ml/5ml
货号	mIHC相关单组分	规格
BDAA0205	350标记Tyramide	2ml/5ml/10ml
BDAA0206	488标记Tyramide	2ml/5ml/10ml
BDAA0207	525标记Tyramide	2ml/5ml/10ml
BDAA0208	555标记Tyramide	2ml/5ml/10ml
BDAA0209	594标记Tyramide	2ml/5ml/10ml
BDAA0210	647标记Tyramide	2ml/5ml/10ml
BDAA0211	680标记Tyramide	2ml/5ml/10ml
BDAA0212	Tyramide稀释液	10ml
BDAA0213	DAPI/封片二合一溶液(防淬灭）	5ml
BDAA0214	抗体剥离液20X	30ml/100ml/200ml
BDAA0215	脱蜡修复二合一溶液20X pH6.0	30ml/100ml/250ml
BDAA0216	脱蜡修复二合一溶液20X pH9.0	30ml/100ml/250ml
BDAA0167	Polymer HRP Goat Anti Mouse/Rabbit IgG(H+L),Ready to Use	3ml/10ml/100ml
BDAA0217	435标记Tyramide	2ml/5ml/10ml
BDAA0218	750标记Tyramide	2ml/5ml/10ml

点击查询更多→https://www.biodragon.cn/cn/goods/product?CatalogId=68

示例：
货号：BDAA0200
名称：鼠兔三标四色荧光检测试剂盒(pH9.0)
试剂盒组分：

名称	名称	规格			保存条件
		1mL	2mL	5mL
试剂A	脱蜡修复二合一溶液 20X	60mL	60mL	200mL	2-8℃
试剂B	过氧化物酶封闭缓冲液	1mL	2mL	5mL	2-8℃
试剂C	HRP 多聚合抗兔/鼠二抗	2mL	4mL	10mL	2-8℃
试剂D-488	488 标记 Tyramide	1mL	2mL	5mL	2-8℃
试剂D-594	594 标记 Tyramide	1mL	2mL	5mL	2-8℃
试剂D-647	647标记 Tyramide	1mL	2mL	5mL	2-8℃
试剂G	抗淬灭DAPI 封片二合一溶液	1mL	2mL	5mL	2-8℃
试剂F	抗体剥离液 20X	60mL	60mL	200mL	2-8℃

Fluorescence multiplex immunohistochemical analysis of normal human appendix tissue (formalin-fixed paraffin-embedded section).
The section was incubated in 3 rounds of staining; in the order of CK PAN .( BD-PM6815； 1/200 dilution; red), PD-1.(BD-PM6208; 1/200 dilution; purple), Caldesmon pan. (BD-PM6826;  1/200 dilution; green); each using a separate fluorescent tyramide signal amplification system : [Treble-Fluorescence immunohistochemical mouse/rabbit kit] Catalog NO: BDAA0200, Biodragon.