产品基本信息

• 产品货号 BD-PB5132
• 别名 Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC, ARPC5, ARC16
• 产品名称 ARPC5 Rabbit Polyclonal Antibody (Center)
• 类别 抗原抗体
• Clonality Polyclonal
• 推荐应用 WB, IF-ICC
• 反应种属 Human, Rat, Mouse
• 存储缓冲液 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
• 特异性 This ARPC5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 67-101 amino acids from the Central region of human ARPC5.
• 稀释度 IF-ICC 1:25, WB 1:2000
• 预测分子量 16kDa
• 运输及保存条件 Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• 宿主 Rabbit
• 同种型 IgG
• 背景介绍 Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
• 细胞定位 Cytoplasm, cytoskeleton. Cell projection. Nucleus
• 功能 Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).

图片

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling ARPC5 with BD-PB5132 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

All lanes : Anti-ARPC5 Antibody (Center) at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: Human spleen lysate Lane 6: Mouse brain lysate Lane 7: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情