产品基本信息

• 产品货号 BD-PB4645
• 别名 Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, CRF2-1, Type I interferon receptor 1, IFNAR1, IFNAR
• 产品名称 IFNAR1 Rabbit Polyclonal Antibody (Center)
• 类别 抗原抗体
• Clonality Polyclonal
• 推荐应用 WB, FCM, IHC-P-Leica
• 反应种属 Human, Rat, Mouse
• 存储缓冲液 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Human Gene ID NP_000620.2
• Human Swissprot No. P17181
• 特异性 This IFNAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-188 amino acids of human IFNAR1.
• 稀释度 IHC-P-Leica 1:1000, FCM 1:25, WB 1:1000
• 预测分子量 64kDa
• 运输及保存条件 Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• 宿主 Rabbit
• 同种型 IgG
• 背景介绍 IFNAR1 is the receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha-and beta-subunits themselves.
• 组织表达 IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN- alpha resistant myeloma cell line U266R.
• 细胞定位 [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Late endosome. Lysosome Note=Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes
• 功能 Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986). Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves (PubMed:7665574, PubMed:21854986, PubMed:32972995). Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).
• 期货 现货

图片

	Immunohistochemical analysis of paraffin-embedded human brain tissue using BD-PB4645 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
	Overlay histogram showing K562 cells stained with BD-PB4645(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
	All lanes : Anti-IFNAR1 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: human brain lysate Lane 5: human heart lysate Lane 6: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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