产品基本信息

• 产品货号 BD-PB4191
• 别名 G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
• 产品名称 CCND1 (18J4) Mouse Monoclonal Antibody
• 类别 抗原抗体
• Clonality Monoclonal
• 推荐应用 WB
• 反应种属 Human, Rat, Mouse
• Human Swissprot No. P24385
• 特异性 This antibody is generated from a mouse immunized with a recombinant protein from human.
• 稀释度 WB 1:2000
• 预测分子量 34kDa
• 同种型 IgG1,κ
• 背景介绍 Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
• 细胞定位 Nucleus. Cytoplasm Nucleus membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members
• 期货 现货

图片

	All lanes : Anti-CCND1 at 1:2000 dilution Lane 1: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size : 33kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	All lanes : Anti-CCND1 at dilution Lane 1: MCF-7 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: L929 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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