产品基本信息

• 产品货号 BD-PB3860
• 别名 Transcription factor SOX-2, SOX2
• 产品名称 SOX2 (17V16) Mouse Monoclonal Antibody
• 类别 抗体产品
• Clonality Monoclonal
• 推荐应用 IF-ICC, WB, IHC-P, FCM
• 反应种属 Human, Mouse
• Human Gene ID NP_003097.1
• Human Swissprot No. P48431
• 特异性 SOX2 recombinant protein is used to produce this monoclonal antibody.
• 稀释度 WB 1:200-2000, IF-ICC 1:100, IHC-P 1:100-500, FCM 1:10-50, IHC 1:100
• 预测分子量 34kDa
• 同种型 IgG1
• 背景介绍 This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
• 细胞定位 Nucleus {ECO:0000250|UniProtKB:P48432}.

图片

	Western blot analysis of lysate from SOX2 protein, using SOX2 Antibodyat 1:3000 dilution was used as the secondary antibody. Lysate at 20μg.
	Fluorescent image of A549 cell stained with SOX2 Antibodywas used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.SOX2 immunoreactivity is localized to Nucleus significantly.
	Western blot analysis of SOX2 Antibody by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.
	Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2)
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
	Immunohistochemical analysis of paraffin-embedded Human Lung squamous cell carcinoma section using Pink1. BD-PB3860 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

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