产品基本信息
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产品货号
RM4578
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别名
ERN1, ER to nucleus signalling 1, Inositol-requiring enzyme 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, HIRE1p, IRE1, Inositol-requiring 1, IRE1P;
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产品名称
IRE1 (Phospho S724) (4K17) Rabbit Monoclonal Antibody
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类别
抗原抗体
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基因名称
ERN1
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Clonality
Monoclonal
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推荐应用
WB
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反应种属
Human
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存储缓冲液
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
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Human Gene ID
2081
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免疫原
A synthetic phosphopeptide corresponding to residues surrounding
Ser724 of human IRE1
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稀释度
WB 1:1000-1:5000
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参考分子量
110kDa
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预测分子量
110kDa
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运输及保存条件
-20°C/1 year
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宿主
Rabbit
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同种型
IgG
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注意事项
Phospho-IRE1 (S724) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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组织表达
Ubiquitously expressed. High levels observed in pancreatic tissue.
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细胞定位
Endoplasmic reticulum membrane; Single-pass type I membrane protein
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功能
Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875, PubMed:28128204). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed:21884936, PubMed:28067262).
图片
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Western blot detection of IRE1 (Phospho-Ser724) in K562 cell lysates using IRE1 (Phospho-Ser724) antibody(1:1000 diluted). |
产品详情
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.