产品基本信息

• 产品货号 RM4578
• 别名 ERN1, ER to nucleus signalling 1, Inositol-requiring enzyme 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, HIRE1p, IRE1, Inositol-requiring 1, IRE1P;
• 产品名称 IRE1 (Phospho S724) (4K17) Rabbit Monoclonal Antibody
• 类别 抗原抗体
• 基因名称 ERN1
• Clonality Monoclonal
• 推荐应用 WB
• 反应种属 Human
• 存储缓冲液 Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
• Human Gene ID 2081
• 免疫原 A synthetic phosphopeptide corresponding to residues surrounding Ser724 of human IRE1
• 稀释度 WB 1:1000-1:5000
• 参考分子量 110kDa
• 预测分子量 110kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 IgG
• 注意事项 Phospho-IRE1 (S724) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• 组织表达 Ubiquitously expressed. High levels observed in pancreatic tissue.
• 细胞定位 Endoplasmic reticulum membrane; Single-pass type I membrane protein
• 功能 Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875, PubMed:28128204). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed:21884936, PubMed:28067262).

图片

Western blot detection of IRE1 (Phospho-Ser724) in K562 cell lysates using IRE1 (Phospho-Ser724) antibody(1:1000 diluted).

产品详情