Microtiter wells were coated with BDAM0027 at 3 µg/mL as the capture antibody. EGF was used as the antigen. Peroxidase conjugated mouse anti-human EGF monoclonal antibody (BDAM0024) was used as the detection antibody. Result: BDAM0024 and BDAM0027 can be used as a matched antibody pair to detect and quantify the concentration of EGF. |