组分名称 | 货号 | ||
BDAG0052-1ml | BDAG0052-5ml | BDAG0052-10ml | |
2×Mouse Tissue Direct TaqMan qPCR Mix | 1 mL | 5 mL | 10 mL |
Buffer TLB03 | 20 mL | 100 mL | 2,000 mL |
ROX ReferenceⅠ (50×) * | 40 µL | 200 µL | 400 µL |
ROX ReferenceⅡ (50×) * | 40 µL | 200 µL | 400 µL |
组分 | 体积 (µL per sample) | 终浓度 | |
Mix A | Mix B | ||
2×Mouse Tissue Direct TaqMan qPCR Mix Forward Primer (10 μM) Reverse Primer (10 μM) Probe (10 μM) Template* PCR Grade H2O |
10 0.5 0.5 0.4 2 To 20 |
25 1.0 1.0 0.4 5 To 50 |
1× 0.25 μM 0.25 μM 0.2 μM / / |
总体积/rxn | 20 | 50 |
Step | Temp. | Time | Cycle |
Initial Denaturation | 95℃ | 5 min | 1 Cycle |
Denaturation Annealing / Extension / Data Collection |
95℃ 60℃ |
15 sec 30 sec |
35~45 Cycles |
组分名称 | 货号 | ||
BDAG0052-1ml | BDAG0052-5ml | BDAG0052-10ml | |
2×Mouse Tissue Direct TaqMan qPCR Mix | 1 mL | 5 mL | 10 mL |
Buffer TLB03 | 20 mL | 100 mL | 2,000 mL |
ROX ReferenceⅠ (50×) * | 40 µL | 200 µL | 400 µL |
ROX ReferenceⅡ (50×) * | 40 µL | 200 µL | 400 µL |
组分 | 体积 (µL per sample) | 终浓度 | |
Mix A | Mix B | ||
2×Mouse Tissue Direct TaqMan qPCR Mix Forward Primer (10 μM) Reverse Primer (10 μM) Probe (10 μM) Template* PCR Grade H2O |
10 0.5 0.5 0.4 2 To 20 |
25 1.0 1.0 0.4 5 To 50 |
1× 0.25 μM 0.25 μM 0.2 μM / / |
总体积/rxn | 20 | 50 |
Step | Temp. | Time | Cycle |
Initial Denaturation | 95℃ | 5 min | 1 Cycle |
Denaturation Annealing / Extension / Data Collection |
95℃ 60℃ |
15 sec 30 sec |
35~45 Cycles |