产品基本信息

• 产品货号 BD-PB3382
• 别名 Protein lin-28 homolog A, Lin-28A, lin28a, lin28
• 产品名称 (DANRE) lin28a Rabbit Polyclonal Antibody (Center)
• 类别 抗原抗体
• 推荐应用 WB
• 反应种属 Zebrafish
• 特异性 This DANRE lin28a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 85-119 amino acids from the Central region of DANRE lin28a.
• 稀释度 WB 1:2000
• 预测分子量 22kDa
• 同种型 Rabbit Ig
• 背景介绍 Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting an uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation (By similarity).
• 细胞定位 Cytoplasm {ECO:0000250|UniProtKB:Q8K3Y3}. Rough endoplasmic reticulum {ECO:0000250|UniProtKB:Q8K3Y3}. Cytoplasm, P-body {ECO:0000250|UniProtKB:Q9H9Z2}. Cytoplasm, Stress granule {ECO:0000250|UniProtKB:Q8K3Y3}. Nucleus, nucleolus {ECO:0000250|UniProtKB:Q8K3Y3}. Note=Predominantly cytoplasmic. In the cytoplasm, localizes to peri-endoplasmic reticulum regions and may be bound to the cytosolic surface of rough endoplasmic reticulum (ER) on which ER-associated mRNAs are translated. Shuttle from the nucleus to the cytoplasm requires RNA-binding. {ECO:0000250|UniProtKB:Q8K3Y3}

图片

All lanes : Anti-(DANRE) lin28a Antibody (Center) at 1:2000 dilution Lane 1: Zebrafish muscle lysates Lane 2: Zebrafish lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情