产品基本信息

• 产品货号 BD-PB1999
• 别名 Doublesex- and mab-3-related transcription factor A2, Doublesex- and mab-3-related transcription factor 5, DMRTA2, DMRT5
• 产品名称 DMRTA2 Rabbit Polyclonal Antibody (C-term)
• 类别 抗原抗体
• 推荐应用 IF-ICC, WB
• 反应种属 Human, Zebrafish, Mouse
• Human Gene ID NP_115486.1
• Human Swissprot No. Q96SC8
• 特异性 This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
• 稀释度 WB 1:1000, IF-ICC 1:10-50
• 预测分子量 53kDa
• 同种型 Rabbit Ig
• 背景介绍 DMRTA2 may be involved in sexual development.
• 组织表达 Expressed in testis..
• 细胞定位 Nucleus {ECO:0000255|PROSITE-ProRule:PRU00070}.
• 纯化 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

图片

DMRTA2 Antibody (C-term) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).

Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.

产品详情