产品基本信息

• 产品货号 BD-PB0935
• 别名 Round spermatid basic protein 1, RSBN1
• 产品名称 RSBN1 Rabbit Polyclonal Antibody (N-term)
• 类别 抗原抗体
• Clonality Polyclonal
• 推荐应用 WB, IHC-P, FCM
• 反应种属 Human, Mouse
• Human Gene ID NP_060834.2
• 特异性 This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1.
• 稀释度 IHC-P 1:100-500, WB 1:2000
• 预测分子量 90kDa
• 宿主 Rabbit
• 同种型 IgG
• 细胞定位 Nucleus.
• 纯化 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

图片

BD-PB0935 staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human liver lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 90 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情