All lanes : Anti-DANRE PAX9 Antibody at 1:1000 dilution Lane 1: zebrafish muscle lysate Lane 2: zebrafish lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |