产品基本信息
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产品货号
BD-PA0006
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别名
GSK-3β; GSK3-beta
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产品名称
GSK3B Mouse Monoclonal Antibody
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类别
抗原抗体
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基因名称
GSK3B
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Clonality
Monoclonal
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推荐应用
WB,IHC-P,ICC,FCM,Elisa
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反应种属
Human,Mouse,Monkey,Rat
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存储缓冲液
Ascitic fluid containing 0.03% New type preservative N.
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Human Gene ID
2932
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免疫原
Purified recombinant fragment of human GSK3B expressed in E. Coli.
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稀释度
WB 1:500-1:2000, IHC-P 1:200-1:1000, ICC 1:200-1:1000, FCM 1:200-1:400, ELISA 1:10000
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参考分子量
46kDa
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运输及保存条件
4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
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宿主
Mouse
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同种型
IgG2a
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克隆号
3D10
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期货
3个工作日
图片
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Western blot analysis using GSK3B mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate. |
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Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. |
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Flow cytometric analysis of Hela cells using GSK3B mouse mAb (green) and negative control (purple). |
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Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mouse mAb with DAB staining. |
产品详情
Glycogen synthase kinase 3 (GSK-3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK-3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK-3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK-3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK-3 are ubiquitously expressed, although particularly high levels of GSK-3beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK-3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3
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