产品基本信息

• 产品货号 BD-PT5493
• 别名 UBA1; A1S9T; UBE1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; Ubiquitin-activating enzyme E1
• 产品名称 UBA1 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 UBA1
• 蛋白名称 Ubiquitin-like modifier-activating enzyme 1
• Clonality Polyclonal
• 推荐应用 WB,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 7317
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7317
• Human Swissprot No. P22314
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P22314/entry
• Mouse Gene ID 22201
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22201
• Mouse Swissprot No. Q02053
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q02053
• Rat Gene ID 314432
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=314432
• Rat Swissprot No. Q5U300
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q5U300
• 免疫原 The antiserum was produced against synthesized peptide derived from the N-terminal region of human UBA1. AA range:91-140
• 特异性 UBA1 Polyclonal Antibody detects endogenous levels of UBA1 protein.
• 稀释度 WB 1:500-1:2000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 118kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008],
• 组织表达 Detected in erythrocytes (at protein level). Ubiquitous.
• 细胞定位 Cytoplasm . Mitochondrion . Nucleus .; [Isoform 1]: Nucleus .; [Isoform 2]: Cytoplasm .
• 信号通路 Ubiquitin mediated proteolysis;Parkinson's disease;
• 功能 disease:Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.,function:Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.,miscellaneous:There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Monomer (By similarity). Interacts with GAN (via BTB domain).,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

Western Blot analysis of PC12 cells using UBA1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

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