产品基本信息

• 产品货号 BD-PT3835
• 别名 PPARA; NR1C1; PPAR; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1
• 产品名称 PPAR-α Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 PPARA
• 蛋白名称 Peroxisome proliferator-activated receptor alpha
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 5465
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5465
• Human Swissprot No. Q07869
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q07869/entry
• Mouse Gene ID 19013
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19013
• Mouse Swissprot No. P23204
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P23204
• Rat Gene ID 25747
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25747
• Rat Swissprot No. P37230
• Rat Swissprot Link http://www.uniprot.org/uniprot/P37230
• 免疫原 The antiserum was produced against synthesized peptide derived from human PPAR-alpha. AA range:6-55
• 特异性 PPAR-α Polyclonal Antibody detects endogenous levels of PPAR-α protein.
• 稀释度 WB 1:500-2000, ELISA 1:10000-20000, IHC-P 1:50-300, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 52kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 peroxisome proliferator activated receptor alpha(PPARA) Homo sapiens Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for thi
• 组织表达 Skeletal muscle, liver, heart and kidney. Expressed in monocytes (PubMed:28167758).
• 细胞定位 Nucleus.
• 信号通路 PPAR;Adipocytokine;
• 功能 function:Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids.,online information:Peroxisome proliferator-activated receptor entry,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Heterodimer with the retinoid X receptor. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Also interacts with PPARBP coactivator in vitro. Interacts with AKAP13.,tissue specificity:Skeletal muscle, liver, heart and kidney.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of HeLa cells, using PPAR-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from NIH/3T3 cells, using PPAR-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
	Immunofluorescence analysis of rat-lung tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-lung tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-spleen tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-spleen tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of mouse-kidney tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of mouse-kidney tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,PPAR-α Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,PPAR-α Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,PPAR-α Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Western Blot analysis of 3T3 cells using PPAR-α Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

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