产品基本信息

• 产品货号 BD-PT3091
• 别名 NFYB; HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB
• 产品名称 NF-YB Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 NFYB
• 蛋白名称 Nuclear transcription factor Y subunit beta
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 4801
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4801
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P25208/entry
• Mouse Gene ID 18045
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18045
• Mouse Swissprot No. P63139
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P63139
• Rat Gene ID 25336
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25336
• Rat Swissprot No. P63140
• Rat Swissprot Link http://www.uniprot.org/uniprot/P63140
• 免疫原 The antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50
• 特异性 NF-YB Polyclonal Antibody detects endogenous levels of NF-YB protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 29kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008],
• 组织表达 Urinary bladder,
• 细胞定位 Nucleus.
• 信号通路 Antigen processing and presentation;
• 功能 domain:Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.,function:Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.,similarity:Belongs to the NFYB/HAP3 subunit family.,subunit:Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of lysates from 293 cells, using NFYB Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of various cells using NF-YB Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.
	Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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