产品基本信息

• 产品货号 BD-PT2928
• 别名 MYD88; Myeloid differentiation primary response protein MyD88
• 产品名称 MyD88 Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 MyD88
• 蛋白名称 Myeloid differentiation primary response protein MyD88
• Clonality Polyclonal
• 推荐应用 IF-P,IF-F,IF-ICC,WB,IHC-P,ELISA
• 反应种属 Human,Mouse,Rat,Pig
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 4615
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4615
• Human Swissprot No. Q99836
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q99836/entry
• Mouse Gene ID 17874
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17874
• Mouse Swissprot No. P22366
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P22366
• Rat Gene ID 301059
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=301059
• Rat Swissprot No. Q6Y1S1
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q6Y1S1
• 免疫原 The antiserum was produced against synthesized peptide derived from human MyD88. AA range:171-220
• 特异性 MyD88 Polyclonal Antibody detects endogenous levels of MyD88 protein.
• 稀释度 IF-P/IF-F/IF-ICC 1:50-200, WB 1:500-1:2000, IHC-P 1:100-1:300, Immunocytochemistry 1:200-1:1000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 33kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010],
• 组织表达 Ubiquitous.
• 细胞定位 Cytoplasm . Nucleus .
• 信号通路 Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Toll_Like;
• 功能 disease:Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also called recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.,function:Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. May be involved in myeloid differentiation.,similarity:Contains 1 death domain.,similarity:Contains 1 TIR domain.,subunit:Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1 and IRAK2 via their respective TIR domains. Interacts with IL1RL1.,tissue specificity:Ubiquitous.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of MyD88 antibody in paraffin-embedded human brain tissue.
	Western blot analysis of lysate from COLO cells, using MyD88 antibody.
	Immunofluorescence analysis of mouse-spleen tissue. 1,MyD88 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-lung tissue. 1,MyD88 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,MyD88 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,MyD88 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,MyD88 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Western Blot analysis of various cells using MyD88 Polyclonal Antibody diluted at 1：2000
	Western Blot analysis of COLO205 cells using MyD88 Polyclonal Antibody diluted at 1：2000
	Western blot analysis of mouse-liver mouse-heart mouse-lung Cell Lysate, antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
	Western Blot analysis

产品详情