产品基本信息

• 产品货号 BD-PT2916
• 别名 TFAM; TCF6; TCF6L2; Transcription factor A; mitochondrial; mtTFA; Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2
• 产品名称 mtTFA Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 TFAM
• 蛋白名称 Transcription factor A mitochondrial
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Rat,Mouse,,Bovine
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 7019
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7019
• Human Swissprot No. Q00059
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q00059/entry
• Mouse Swissprot No. P40630
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P40630
• 免疫原 The antiserum was produced against synthesized peptide derived from human TFAM. AA range:131-180
• 特异性 mtTFA Polyclonal Antibody detects endogenous levels of mtTFA protein.
• 稀释度 WB 1:500-2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 22kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012],
• 组织表达 Lymphocyte,Pooled,Uterus,
• 细胞定位 Mitochondrion . Mitochondrion matrix, mitochondrion nucleoid .
• 信号通路 Stem cell pathway; Protein_Acetylation
• 功能 function:Involved in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA.,similarity:Contains 2 HMG box DNA-binding domains.,subunit:Interacts with TFB1M and TFB2M.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of HepG2 cells, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of 293T 3T3 lysis using mtTFA antibody. Antibody was diluted at 1:500
	Immunofluorescence analysis of Hela cell. 1,mtTFA Polyclonal Antibody(Green) was diluted at 1:200(4° overnight). Histone H3 (Tri Methyl Lys36) Monoclonal Antibody (Red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

产品详情