产品基本信息

• 产品货号 BD-PT2694
• 别名 ME2; NAD-dependent malic enzyme; mitochondrial; NAD-ME; Malic enzyme 2
• 产品名称 ME2 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 ME2
• 蛋白名称 NAD-dependent malic enzyme mitochondrial
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 4200
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4200
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P23368/entry
• Mouse Swissprot No. Q99KE1
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q99KE1
• 免疫原 The antiserum was produced against synthesized peptide derived from human ME2. AA range:201-250
• 特异性 ME2 Polyclonal Antibody detects endogenous levels of ME2 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 65kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009],
• 组织表达 B-cell,Placenta,Platelet,
• 细胞定位 Mitochondrion matrix .
• 信号通路 Pyruvate metabolism;
• 功能 catalytic activity:(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.,cofactor:Divalent metal cations. Prefers magnesium or manganese.,enzyme regulation:Subject to allosteric activation by fumarate.,miscellaneous:This isoenzyme can also use NADP(+) but is more effective with NAD(+).,similarity:Belongs to the malic enzymes family.,subunit:Homotetramer.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using ME2 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from RAW264.7 and LOVO cells, using ME2 Antibody. The lane on the right is blocked with the synthesized peptide.

产品详情