产品基本信息

• 产品货号 BD-PT2195
• 别名 HNRNPC; HNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2
• 产品名称 hnRNP C1/C2 Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 HNRNPC
• 蛋白名称 Heterogeneous nuclear ribonucleoproteins C1/C2
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 3183
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3183
• Human Swissprot No. P07910
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P07910/entry
• Mouse Gene ID 15381
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=15381
• Mouse Swissprot No. Q9Z204
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9Z204
• Rat Swissprot No. P17132
• Rat Swissprot Link http://www.uniprot.org/uniprot/P17132
• 免疫原 The antiserum was produced against synthesized peptide derived from human hnRNP C1/C2. AA range:241-290
• 特异性 hnRNP C1/C2 Polyclonal Antibody detects endogenous levels of hnRNP C1/C2 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 33kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008],
• 组织表达 Blood vessels- blood vessel,Bone marrow,Brain,Cajal
• 细胞定位 Nucleus. Component of ribonucleosomes.
• 信号通路 Spliceosome;
• 功能 function:Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.,PTM:Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.,PTM:Sumoylated. Sumoylation reduces affinity for mRNA.,similarity:Belongs to the RRM HNRPC family. RALY subfamily.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Component of ribonucleosomes.,subunit:Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human brain tissue, using hnRNP C1/C2 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of the lysates from HT-29 cells using hnRNP C1/C2 antibody.
	Western Blot analysis of various cells using hnRNP C1/C2 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.
	Immunofluorescence analysis of Siha cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500(room temperature, 50min).

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