产品基本信息

• 产品货号 BD-PT2147
• 别名 HDAC6; KIAA0901; JM21; Histone deacetylase 6; HD6
• 产品名称 Histone deacetylase 6 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 HDAC6
• 蛋白名称 Histone deacetylase 6
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 10013
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10013
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q9UBN7/entry
• Mouse Gene ID 15185
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=15185
• Mouse Swissprot No. Q9Z2V5
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9Z2V5
• 免疫原 The antiserum was produced against synthesized peptide derived from human HDAC6. AA range:1166-1215
• 特异性 Histone deacetylase 6 Polyclonal Antibody detects endogenous levels of Histone deacetylase 6 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 135kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008],
• 组织表达 Brain,Epithelium,Kidney,Muscle,Ovary,Placenta,
• 细胞定位 Cytoplasm . Cytoplasm, cytoskeleton . Nucleus . Perikaryon . Cell projection, dendrite . Cell projection, axon . It is mainly cytoplasmic, where it is associated with microtubules. .
• 信号通路 Protein_Acetylation
• 功能 catalytic activity:Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.,function:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.,PTM:Sumoylated in vitro.,PTM:Ubiquitinated. Its polyubiquitination however does not lead to its degradation.,similarity:Belongs to the histone deacetylase family. Type 2 subfamily.,similarity:Contains 1 UBP-type zinc finger.,subcellular location:It is mainly cytoplasmic, where it is associated with microtubules.,subunit:Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using HDAC6 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from HeLa cells, using HDAC6 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of MCF7 cells using Histone deacetylase 6 Polyclonal Antibody diluted at 1：2000

产品详情