产品基本信息

• 产品货号 BD-PT1443
• 别名 E2F2; Transcription factor E2F2; E2F-2
• 产品名称 E2F-2 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 E2F2
• 蛋白名称 Transcription factor E2F2
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1870
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1870
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q14209/entry
• Mouse Gene ID 242705
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=242705
• Mouse Swissprot No. P56931
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P56931
• 免疫原 The antiserum was produced against synthesized peptide derived from human E2F2. AA range:221-270
• 特异性 E2F-2 Polyclonal Antibody detects endogenous levels of E2F-2 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 48kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits
• 组织表达 Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
• 细胞定位 Nucleus.
• 信号通路 Stem cell pathway; Cell_Cycle_G1S;Cell_Cycle_G2M_DNA; Protein_Acetylation
• 功能 function:Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.,PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP.,tissue specificity:Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of lysates from HepG2, K562, and COLO205 cells, using E2F2 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of various cells using E2F-2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.
	Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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