产品基本信息

• 产品货号 BD-PT1288
• 别名 DAPK2; Death-associated protein kinase 2; DAP kinase 2; DAP-kinase-related protein 1; DRP-1
• 产品名称 DAPK2 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 DAPK2
• 蛋白名称 Death-associated protein kinase 2
• Clonality Polyclonal
• 推荐应用 IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 23604
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23604
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q9UIK4/entry
• Mouse Gene ID 13143
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13143
• Mouse Swissprot No. Q8VDF3
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q8VDF3
• 免疫原 The antiserum was produced against synthesized peptide derived from human DAPK2. AA range:284-333
• 特异性 DAPK2 Polyclonal Antibody detects endogenous levels of DAPK2 protein.
• 稀释度 IHC-P 1:100-1:300, ELISA 1:5000, IF-P/IF-F/IF-ICC 1:50-200
• 参考分子量 43kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008],
• 组织表达 Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.
• 细胞定位 Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
• 信号通路 Pathways in cancer;Bladder cancer;
• 功能 catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-318.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.,tissue specificity:Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using DAPK2 Antibody. The picture on the right is blocked with the synthesized peptide.
	Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.
	Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

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