产品基本信息

• 产品货号 BD-PT1274
• 别名 KRT8; CYK8; Keratin; type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
• 产品名称 Cytokeratin 8 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 KRT8
• 蛋白名称 Keratin type II cytoskeletal 8
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 3856
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3856
• Human Swissprot No. P05787
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P05787/entry
• Mouse Gene ID 16691
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16691
• Mouse Swissprot No. P11679
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P11679
• Rat Gene ID 25626
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25626
• Rat Swissprot No. Q10758
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q10758
• 免疫原 The antiserum was produced against synthesized peptide derived from human Keratin 8. AA range:434-483
• 特异性 Cytokeratin 8 Polyclonal Antibody detects endogenous levels of Cytokeratin 8 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:40000.Not yet tested in other applications.
• 预测分子量 55kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 keratin 8(KRT8) Homo sapiens This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],
• 组织表达 Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
• 细胞定位 Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .
• 功能 disease:Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].,function:Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.,tissue specificity:Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from SKOV3 cells, using Keratin 8 Antibody. The lane on the right is blocked with the synthesized peptide.
	Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.

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