产品基本信息

• 产品货号 BD-PT1244
• 别名 CYSLTR1; CYSLT1; Cysteinyl leukotriene receptor 1; CysLTR1; Cysteinyl leukotriene D4 receptor; LTD4 receptor; G-protein coupled receptor HG55; HMTMF81
• 产品名称 CysLTR1 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CYSLTR1
• 蛋白名称 Cysteinyl leukotriene receptor 1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Monkey
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 10800
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10800
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q9Y271/entry
• Mouse Swissprot No. Q99JA4
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q99JA4
• 免疫原 The antiserum was produced against synthesized peptide derived from human CLTR1. AA range:131-180
• 特异性 CysLTR1 Polyclonal Antibody detects endogenous levels of CysLTR1 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:10000.Not yet tested in other applications.
• 预测分子量 36kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for cysteinyl leukotrienes, and is involved in mediating bronchoconstriction via activation of a phosphatidylinositol-calcium second messenger system. Activation of the encoded receptor results in contraction and proliferation of bronchial smooth muscle cells, eosinophil migration, and damage to the mucus layer in the lung. Upregulation of this gene is associated with asthma and dysregulation may also be implicated in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013],
• 组织表达 Widely expressed, with highest levels in spleen and peripheral blood leukocytes. Lower expression in several tissues, such as lung (mostly in smooth muscle bundles and alveolar macrophages), placenta, small intestine, pancreas, colon and heart.
• 细胞定位 Cell membrane; Multi-pass membrane protein.
• 信号通路 Calcium;Neuroactive ligand-receptor interaction;
• 功能 function:Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.,miscellaneous:Selective antagonists, such as montelukast (Singulair), zafirlukast (Accolate) and pranlukast (Onon), are used in the treatment of the asthma crisis.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Widely expressed, with highest levels in spleen and peripheral blood leukocytes. Lower expression in several tissues, such as lung (mostly in smooth muscle bundles and alveolar macrophages), placenta, small intestine, pancreas, colon and heart.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of HUVEC cells, using CLTR1 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from COS7 cells, using CLTR1 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of COS-7 cells using CysLTR1 Polyclonal Antibody
	Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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