产品基本信息

• 产品货号 BD-PT1242
• 别名 CYP8B1; CYP12; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase
• 产品名称 CYP8B1 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CYP8B1
• 蛋白名称 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1582
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1582
• Human Swissprot No. Q9UNU6
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q9UNU6/entry
• Mouse Gene ID 13124
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13124
• Mouse Swissprot No. O88962
• Mouse Swissprot Link http://www.uniprot.org/uniprot/O88962
• 免疫原 The antiserum was produced against synthesized peptide derived from human Cytochrome P450 8B1. AA range:371-420
• 特异性 CYP8B1 Polyclonal Antibody detects endogenous levels of CYP8B1 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 58kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008],
• 组织表达 Liver.
• 细胞定位 Endoplasmic reticulum membrane ; Single-pass membrane protein . Microsome membrane ; Single-pass membrane protein .
• 信号通路 Primary bile acid biosynthesis;PPAR;
• 功能 catalytic activity:7-alpha-hydroxycholest-4-en-3-one + NADPH + O(2) = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP(+) + H(2)O.,cofactor:Heme group.,function:Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of Cytochrome P450 8B1 Antibody. The lane on the right is blocked with the Cytochrome P450 8B1 peptide.
	Immunohistochemistryt analysis of paraffin-embedded human liver, using Cytochrome P450 8B1 Antibody. The lane on the right is blocked with the Cytochrome P450 8B1 peptide.
	Western blot analysis of the lysates from HT-29 cells using Cytochrome P450 8B1 antibody.
	Western Blot analysis of various cells using CYP8B1 Polyclonal Antibody

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