产品基本信息

• 产品货号 BD-PT1241
• 别名 CYP7B1; 25-hydroxycholesterol 7-alpha-hydroxylase; Cytochrome P450 7B1; Oxysterol 7-alpha-hydroxylase
• 产品名称 CYP7B1 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CYP7B1
• 蛋白名称 25-hydroxycholesterol 7-alpha-hydroxylase
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 9420
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9420
• Human Swissprot No. O75881
• Human Swissprot Link http://www.uniprot.org/uniprotkb/O75881/entry
• Mouse Swissprot No. Q60991
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q60991
• 免疫原 The antiserum was produced against synthesized peptide derived from human Cytochrome P450 7B1. AA range:101-150
• 特异性 CYP7B1 Polyclonal Antibody detects endogenous levels of CYP7B1 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 58kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016],
• 组织表达 Widely expressed. Expressed in brain, testis, ovary, prostate, liver, colon, kidney, small intestine, thymus and spleen.
• 细胞定位 Endoplasmic reticulum membrane ; Multi-pass membrane protein . Microsome membrane ; Multi-pass membrane protein .
• 信号通路 Primary bile acid biosynthesis;Steroid hormone biosynthesis;
• 功能 catalytic activity:Cholest-5-ene-3-beta,25-diol + NADPH + O(2) = cholest-5-ene-3-beta,7-alpha,25-triol + NADP(+) + H(2)O.,catalytic activity:Cholest-5-ene-3-beta,27-diol + NADPH + O(2) = cholest-5-ene-3-beta,7-alpha,27-triol + NADP(+) + H(2)O.,cofactor:Heme group.,disease:Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:603711]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable.,disease:Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.,pathway:Lipid metabolism; bile acid biosynthesis.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Brain, testis, ovary, prostate, liver, colon, kidney, and small intestine.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of lysates from LOVO cells, using Cytochrome P450 7B1 Antibody. The lane on the right is blocked with the synthesized peptide.
	Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.

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