产品基本信息

• 产品货号 BD-PT1209
• 别名 CYP2B6; Cytochrome P450 2B6; 1; 4-cineole 2-exo-monooxygenase; CYPIIB6; Cytochrome P450 IIB1
• 产品名称 CYP2B6 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CYP2B6
• 蛋白名称 Cytochrome P450 2B6
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1555
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1555
• Human Swissprot No. P20813
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P20813/entry
• 免疫原 The antiserum was produced against synthesized peptide derived from human Cytochrome P450 2B6. AA range:241-290
• 特异性 CYP2B6 Polyclonal Antibody detects endogenous levels of CYP2B6 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000.Not yet tested in other applications.
• 预测分子量 56kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008],
• 组织表达 Expressed in liver, lung and heart right ventricle.
• 细胞定位 Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
• 信号通路 Arachidonic acid metabolism;Retinol metabolism;Metabolism of xenobiotics by cytochrome P450;Drug metabolism;
• 功能 catalytic activity:RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.,cofactor:Heme group.,function:Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.,induction:By phenobarbital.,online information:CYP2B6 alleles,PTM:Phosphorylation is accompanied by a decrease in enzyme activity.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Expressed in liver, lung and heart right ventricle.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of lysates from HT-29 cells, using Cytochrome P450 2B6 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of various cells using CYP2B6 Polyclonal Antibody
	Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

产品详情