产品基本信息

• 产品货号 BD-PT1190
• 别名 CYP19A1; ARO1; CYAR; CYP19; Cytochrome P450 19A1; Aromatase; CYPXIX; Cytochrome P-450AROM; Estrogen synthase
• 产品名称 CYP19A1 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CYP19A1
• 蛋白名称 Cytochrome P450 19A1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1588
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1588
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P11511/entry
• Mouse Gene ID 13075
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13075
• Mouse Swissprot No. P28649
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P28649
• Rat Swissprot No. P22443
• Rat Swissprot Link http://www.uniprot.org/uniprot/P22443
• 免疫原 The antiserum was produced against synthesized peptide derived from human Cytochrome P450 19A1. AA range:221-270
• 特异性 CYP19A1 Polyclonal Antibody detects endogenous levels of CYP19A1 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 53kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 cytochrome P450 family 19 subfamily A member 1(CYP19A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
• 组织表达 Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
• 细胞定位 Endoplasmic reticulum membrane ; Multi-pass membrane protein . Microsome membrane ; Multi-pass membrane protein .
• 信号通路 Steroid hormone biosynthesis;Androgen and estrogen metabolism;
• 功能 catalytic activity:RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.,cofactor:Heme group.,disease:Defects in CYP19A1 are a cause of familial gynecomastia [MIM:139300]. This is characterized by an estrogen excess due to an increased aromatase activity.,disease:Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.,function:Catalyzes the formation of aromatic C18 estrogens from C19 androgens.,online information:Aromatase entry,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Brain, placenta and gonads.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Cytochrome P450 19A1 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from 293 cells, using Cytochrome P450 19A1 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western blot analysis of the lysates from HeLa cells using BCOR antibody.
	Western Blot analysis of various cells using CYP19A1 Polyclonal Antibody diluted at 1：1000
	Western Blot analysis of 293 cells using CYP19A1 Polyclonal Antibody diluted at 1：1000
	Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.
	Immunofluorescence analysis of Hela cell. 1,CYP19A1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

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