产品基本信息

• 产品货号 BD-PT1032
• 别名 COL5A2; Collagen alpha-2(V) chain
• 产品名称 COL5A2 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 COL5A2
• 蛋白名称 Collagen alpha-2(V) chain
• Clonality Polyclonal
• 推荐应用 IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1290
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1290
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P05997/entry
• Mouse Swissprot No. Q3U962
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q3U962
• 免疫原 The antiserum was produced against synthesized peptide derived from human Collagen V alpha2. AA range:1-50
• 特异性 COL5A2 Polyclonal Antibody detects endogenous levels of COL5A2 protein.
• 稀释度 IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:40000.Not yet tested in other applications.
• 参考分子量 145kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq, Jul 2008],
• 组织表达 Bone,Brain,Chondrosarcoma,Placenta,Skin,
• 细胞定位 Secreted, extracellular space, extracellular matrix .
• 信号通路 Focal adhesion;ECM-receptor interaction;
• 功能 disease:Defects in COL5A2 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.,disease:Defects in COL5A2 are a cause of Ehlers-Danlos syndrome type 2 (EDS2) [MIM:130010]; also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome.,disease:Genetic variation in COL5A2 is associated with spontaneous cervical artery dissections (sCAD). sCAD are an important cause of stroke among young and middle-aged patients. Ultrastructural abnormalities are observed in skin biopsies of most patients with sCAD. Major findings included enlarged and irregular collagen fibrils and pronounced elastic fibers fragmentation.,function:Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices.,PTM:Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.,similarity:Belongs to the fibrillar collagen family.,similarity:Contains 1 VWFC domain.,subunit:Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of HepG2 cells, using Collagen V alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen V alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.

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