产品基本信息

• 产品货号 BD-PT1001
• 别名 CNOT4; NOT4; CCR4-NOT transcription complex subunit 4; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; Potential transcriptional repressor NOT4Hp
• 产品名称 CNOT4 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CNOT4
• 蛋白名称 CCR4-NOT transcription complex subunit 4
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 4850
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4850
• Human Swissprot Link http://www.uniprot.org/uniprotkb/O95628/entry
• Mouse Gene ID 53621
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=53621
• Mouse Swissprot No. Q8BT14
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q8BT14
• 免疫原 The antiserum was produced against synthesized peptide derived from human CNOT4. AA range:61-110
• 特异性 CNOT4 Polyclonal Antibody detects endogenous levels of CNOT4 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 64kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 CCR4-NOT transcription complex subunit 4(CNOT4) Homo sapiens The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010],
• 组织表达 Mammary gland,Testis,
• 细胞定位 Cytoplasm . Nucleus .
• 信号通路 RNA degradation;
• 功能 function:Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex.,pathway:Protein modification; protein ubiquitination.,PTM:Autoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Sequencing errors.,similarity:Contains 1 C3H1-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. Binds CNOT1 via its C-terminus. Binds E2 ubiquitin ligases via its RING domain.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CNOT4 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from Jurkat and K562 cells, using CNOT4 Antibody. The lane on the right is blocked with the synthesized peptide.

产品详情