产品基本信息

• 产品货号 RM8284
• 别名 Transforming protein RhoA (Rho cDNA clone 12) (h12)
• 产品名称 RhoA (2F13) Rabbit Monoclonal Antibody
• 类别 抗原抗体
• 基因名称 RHOA ARH12 ARHA RHO12
• 蛋白名称 Rho A
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA
• 反应种属 Human,Mouse,Rat
• 存储缓冲液 PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
• Human Gene ID 387
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=387
• Human Swissprot No. P61586
• Human Swissprot Link https://www.uniprot.org/uniprotkb/P61586/entry
• Mouse Gene ID 11848
• Mouse Gene Link https://www.uniprot.org/uniprot/11848
• Mouse Swissprot No. Q9QUI0
• Mouse Swissprot Link https://www.uniprot.org/uniprotkb/Q9QUI0
• Rat Gene ID 117273
• Rat Gene Link https://www.uniprot.org/uniprot/117273
• Rat Swissprot No. P61589
• Rat Swissprot Link https://www.uniprot.org/uniprotkb/P61589
• 稀释度 IHC-P 1:200-1000,WB 1:1000-5000,IF-P/IF-F/IF-ICC 1:200-1000,ELISA 1:5000-20000,IP 1:50-200
• 参考分子量 21kDa
• 预测分子量 21kDa
• 运输及保存条件 -20°C/1 year(Do not lower than -25°C)
• 宿主 Rabbit
• 同种型 Monoclonal, rabbit, IgG, Kappa
• 背景介绍 This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
• 功能 cofactor:Magnesium.,domain:The basic-rich region is essential for yopT recognition and cleavage.,function:Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.,PTM:Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.,PTM:Substrate for botulinum ADP-ribosyltransferase.,similarity:Belongs to the small GTPase superfamily. Rho family.,subunit:Interacts with RGNEF (By similarity). Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation.,
• 期货 现货
• 纯化 Protein A

图片

Western blot analysis of lysates from NIH3T3 cells, using RhoA Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

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