产品基本信息

• 产品货号 BD-PT0744
• 别名 TLR4; Toll-like receptor 4; hToll; CD antigen CD284
• 产品名称 CD284 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 TLR4
• 蛋白名称 Toll-like receptor 4
• Clonality Polyclonal
• 推荐应用 IF-P,IF-F,IF-ICC,WB,IHC-P,ELISA
• 反应种属 Human,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 7099
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7099
• Human Swissprot No. O00206
• Human Swissprot Link http://www.uniprot.org/uniprotkb/O00206/entry
• Mouse Gene ID 21898
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21898
• Mouse Swissprot No. Q9QUK6
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9QUK6
• 免疫原 The antiserum was produced against synthesized peptide derived from human CD284. AA range:392-441
• 特异性 CD284 Polyclonal Antibody detects endogenous levels of CD284 protein.
• 稀释度 IF-P/IF-F/IF-ICC 1:50-200, WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000.Not yet tested in other applications.
• 预测分子量 95kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],
• 组织表达 Highly expressed in placenta, spleen and peripheral blood leukocytes (PubMed:9435236, PubMed:9237759). Detected in monocytes, macrophages, dendritic cells and several types of T-cells (PubMed:9237759, PubMed:27022195).
• 细胞定位 Cell membrane ; Single-pass type I membrane protein . Early endosome . Cell projection, ruffle . Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis (PubMed:20037584). Colocalizes with RFTN1 at cell membrane and then together with RFTN1 moves to endosomes, upon lipopolysaccharide stimulation. .
• 信号通路 Toll_Like;Pathogenic Escherichia coli infection;
• 功能 disease:Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.,domain:The TIR domain mediates interaction with NOX4.,function:Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.,polymorphism:Allele TLR4*B (Gly-299, Ile-399) is associated with a blunted response to inhaled LPS.,PTM:N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.,similarity:Belongs to the Toll-like receptor family.,similarity:Contains 1 TIR domain.,similarity:Contains 21 LRR (leucine-rich) repeats.,subunit:Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4.,tissue specificity:Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of TLR4 antibody in paraffin-embedded human brain tissue.
	Western blot analysis of lysate from HeLa cells, using TLR4 antibody.
	Immunofluorescence analysis of human-stomach tissue. 1,CD284 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of human-stomach tissue. 1,CD284 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of mouse-liver tissue. 1,CD284 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of mouse-spleen tissue. 1,CD284 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,CD284 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,CD284 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Western Blot analysis of various cells using CD284 Polyclonal Antibody diluted at 1：1000
	Western Blot analysis of COLO205 cells using CD284 Polyclonal Antibody diluted at 1：1000

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