产品基本信息

• 产品货号 B2210
• 产品名称 His-Tag (14S16) Rabbit Monoclonal Antibody
• 类别 抗原抗体
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-P,IF-ICC,FCM,IP
• 反应种属 Transfected
• 存储缓冲液 Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
• 免疫原 A synthetic peptide of His tag
• 稀释度 WB 1:1000, IHC-P/IF-P 1:500, IF-ICC 1:100, FCM 1:500, IP 1:20
• 参考分子量 According to the fused protein
• 预测分子量 1kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 IgG
• 背景介绍 Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
• 纯化 Affinity Purification

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产品详情