背景介绍AAV vectors are promising delivery tools for human gene therapy. AAV is a single-stranded DNA parvovirus with a 4.7 kb genome composed of the rep and cap genes flanked by inverted terminal repeats (ITRs). The rep gene encodes non-structural proteins involved in viral replication, packaging, and genomic integration, whereas the cap gene codes for structural proteins (VP1, VP2, VP3) that assemble to form the viral capsid, which serves as the viral gene delivery vehicle.
纯化Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)