产品基本信息

• 产品货号 BD-PT0468
• 别名 BCL10; CIPER; CLAP; B-cell lymphoma/leukemia 10; B-cell CLL/lymphoma 10; Bcl-10; CARD-containing molecule enhancing NF-kappa-B; CARD-like apoptotic protein; hCLAP; CED-3/ICH-1 prodomain homologous E10-like regulator; CIPER; Cellular homolog
• 产品名称 Bcl-10 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 BCL10
• 蛋白名称 B-cell lymphoma/leukemia 10
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 8915
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8915
• Human Swissprot Link http://www.uniprot.org/uniprotkb/O95999/entry
• Mouse Gene ID 12042
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12042
• Mouse Swissprot No. Q9Z0H7
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9Z0H7
• Rat Gene ID 83477
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=83477
• Rat Swissprot No. Q9QYN5
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q9QYN5
• 免疫原 The antiserum was produced against synthesized peptide derived from human BCL10. AA range:111-160
• 特异性 Bcl-10 Polyclonal Antibody detects endogenous levels of Bcl-10 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 36kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],
• 组织表达 Ubiquitous.
• 细胞定位 Cytoplasm, perinuclear region . Membrane raft . Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. .
• 信号通路 T_Cell_Receptor;B_Cell_Antigen;
• 功能 disease:A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.,disease:Defects in BCL10 are involved in various types of cancer.,function:Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.,PTM:Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.,similarity:Contains 1 CARD domain.,subcellular location:Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells.,subunit:Self-associates by CARD-CARD interaction and forms a tight complex with MALT1. Interacts with other CARD-proteins such as CARD9, CARD10, CARD11 and CARD14. Binds caspase-9 with its C-terminal domain. Interacts with TRAF2 and BIRC2/c-IAP2.,tissue specificity:Ubiquitous.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using BCL10 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from K562 cells, using BCL10 Antibody. The lane on the right is blocked with the synthesized peptide.
	Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

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