产品基本信息

• 产品货号 BD-PE1267
• Uniprot ID P02511
• Gene ID NM_001885
• 产品名称 CRYAB (Alpha B Crystallin) (13M10) Mouse Monoclonal antibody
• 类别 抗体产品
• 基因名称 CRYAB
• Clonality Monoclonal
• 推荐应用 IF-P,IF-F,IF-ICC,IHC-P,IHC-F,IP,WB
• 反应种属 Human,Mouse,Rat
• 存储缓冲液 PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
• 免疫原 Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
• 稀释度 WB 1:500, IHC-P/IHC-F 1:50, IF-P/IF-F/IF-ICC 1:100, IP 2ug/500ul
• 预测分子量 20kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Mouse
• 同种型 IgG1
• 背景介绍 Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. [provided by RefSeq]
• 纯化 Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

图片

	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1267)
	Anti-CRYAB mouse monoclonal antibody (BD-PE1267) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB .
	HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB. Positive lysates (100ug) and (20ug) can be purchased separately from biodragon.

产品详情