产品基本信息

• 产品货号 BD-PT0125
• 别名 ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha; ADD2; ADDB; Beta-adducin; Erythrocyte adducin subunit beta
• 产品名称 Adducin α/β Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 ADD1/ADD2
• 蛋白名称 Alpha-adducin/Beta-adducin
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IP,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 118/119
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=118
• Human Swissprot No. P35611/P35612
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P35611/entry
• Mouse Gene ID 11518/11519
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11518
• Rat Gene ID 24170/24171
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24170
• Rat Swissprot No. Q63028/Q05764
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q63028
• 免疫原 The antiserum was produced against synthesized peptide derived from human ADD1. AA range:688-737
• 特异性 Adducin α/β Polyclonal Antibody detects endogenous levels of Adducin α/β protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, Immunoprecipitation 2-5 ug/mg lysate, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:40000.Not yet tested in other applications.
• 预测分子量 80kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],
• 组织表达 Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
• 细胞定位 Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
• 功能 alternative products:Additional isoforms seem to exist,domain:Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.,function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.,PTM:The N-terminus is blocked.,similarity:Belongs to the aldolase class II family. Adducin subfamily.,subunit:Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.,tissue specificity:Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of HeLa cells, using ADD1 Antibody. The picture on the right is blocked with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ADD1 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from HeLa cells, treated with Forskolin 40nM 30‘, using ADD1 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of various cells using Adducin α/β Polyclonal Antibody diluted at 1：1000
	Western Blot analysis of HELA cells using Adducin α/β Polyclonal Antibody diluted at 1：1000

产品详情