产品基本信息

• 产品货号 BD-PT0071
• 别名 ACAT1; ACAT; MAT; Acetyl-CoA acetyltransferase; mitochondrial; Acetoacetyl-CoA thiolase; T2
• 产品名称 ACAT-1 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 ACAT1
• 蛋白名称 Acetyl-CoA acetyltransferase mitochondrial
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 38
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=38
• Human Swissprot No. P24752
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P24752/entry
• Mouse Gene ID 110446
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=110446
• Mouse Swissprot No. Q8QZT1
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q8QZT1
• Rat Gene ID 25014
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25014
• Rat Swissprot No. P17764
• Rat Swissprot Link http://www.uniprot.org/uniprot/P17764
• 免疫原 The antiserum was produced against synthesized peptide derived from human ACAT1. AA range:221-270
• 特异性 ACAT-1 Polyclonal Antibody detects endogenous levels of ACAT-1 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 45kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009],
• 组织表达 Adipocyte,Brain,Fetal brain cortex,
• 细胞定位 Mitochondrion .
• 信号通路 Fatty acid metabolism;Synthesis and degradation of ketone bodies;Valine; leucine and isoleucine degradation;Lysine degradation;Tryptophan metabolism;Pyruvate metabolism;Propanoate metabolism;Butanoate metabolism;Terpenoid backbone biosynthesis;
• 功能 catalytic activity:2 acetyl-CoA = CoA + acetoacetyl-CoA.,disease:Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.,enzyme regulation:Activated by potassium ions, but not sodium ions.,function:Plays a major role in ketone body metabolism.,similarity:Belongs to the thiolase family.,subunit:Homotetramer.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of lysates from HepG2, Jurkat, 293, and A549 cells, using ACAT1 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western blot analysis of the lysates from HepG2 cells using ACAT1 antibody.
	Western Blot analysis of various cells using ACAT-1 Polyclonal Antibody
	Western Blot analysis of A549 cells using ACAT-1 Polyclonal Antibody
	Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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