产品基本信息

• 产品货号 BD-PT0021
• 别名 EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
• 产品名称 4E-BP1 Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 EIF4EBP1
• 蛋白名称 Eukaryotic translation initiation factor 4E-binding protein 1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1978
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1978
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q13541/entry
• Mouse Gene ID 13685
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13685
• Mouse Swissprot No. Q60876
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q60876
• Rat Gene ID 116636
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116636
• Rat Swissprot No. Q62622
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q62622
• 免疫原 The antiserum was produced against synthesized peptide derived from human 4E-BP1. AA range:4-53
• 特异性 4E-BP1 Polyclonal Antibody detects endogenous levels of 4E-BP1 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:5000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 18kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],
• 组织表达 Colon,Epithelium,Lung,Placenta,Platelet,
• 细胞定位 nucleoplasm,cytoplasm,cytosol,protein complex,
• 信号通路 Regulates Angiogenesis; Insulin Receptor; mTOR; ErbB/HER; PI3K/Akt; AMPK
• 功能 function:Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.,PTM:Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eIF4E-binding protein family.,subunit:Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from MDA-MB-435 cells, treated with EGF 200ng/ml 30‘, using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

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