产品基本信息

• 产品货号 BD-PP1032
• 别名 PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1
• 产品名称 PDK1 (phospho Tyr9) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 PDPK1
• 蛋白名称 3-phosphoinositide-dependent protein kinase 1
• Clonality Polyclonal
• 推荐应用 IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 5170
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5170
• Human Swissprot Link http://www.uniprot.org/uniprotkb/O15530/entry
• Mouse Gene ID 18607
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18607
• Mouse Swissprot No. Q9Z2A0
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9Z2A0
• Rat Gene ID 81745
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81745
• Rat Swissprot No. O55173
• Rat Swissprot Link http://www.uniprot.org/uniprot/O55173
• 免疫原 The antiserum was produced against synthesized peptide derived from human PDK1 around the phosphorylation site of Tyr9. AA range:1-50
• 特异性 Phospho-PDK1 (Y9) Polyclonal Antibody detects endogenous levels of PDK1 protein only when phosphorylated at Y9.
• 稀释度 IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 参考分子量 63kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,
• 组织表达 Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.
• 细胞定位 Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non-neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resulting in its translocation to other cellular compartments. The tyrosine-phosphorylated form colocalizes with PTK2B in focal adhesions after angiotensin II stimulation.
• 信号通路 Akt_PKB;PPAR;mTOR;Focal adhesion;Insulin_Receptor;Aldosterone-regulated sodium reabsorption;Endometrial cancer;Prostate cancer;Non-small cell lung cancer;
• 功能 catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using PDK1 （Phospho-Tyr9） Antibody
	Immunohistochemistry analysis of paraffin-embedded human brain, using PDK1 （Phospho-Tyr9） Antibody. The picture on the right is blocked with the phospho peptide.

产品详情