产品基本信息

• 产品货号 BD-PP0889
• 别名 PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C
• 产品名称 PP2A-Cα (phospho Tyr307) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 PPP2CA
• 蛋白名称 Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• Clonality Polyclonal
• 推荐应用 WB,IP,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 5515
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5515
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P67775/entry
• Mouse Gene ID 19052
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19052
• Mouse Swissprot No. P63330
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P63330
• Rat Gene ID 24672
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24672
• Rat Swissprot No. P63331
• Rat Swissprot Link http://www.uniprot.org/uniprot/P63331
• 免疫原 The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307. AA range:260-309
• 特异性 Phospho-PP2A-Cα (Y307) Polyclonal Antibody detects endogenous levels of PP2A-Cα protein only when phosphorylated at Y307.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000.Not yet tested in other applications.
• 预测分子量 35kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008],
• 组织表达 Fibroblast,Liver,Lung,Placenta,Testis,Uterus,
• 细胞定位 Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres (PubMed:16541025). During mitosis, also found at spindle poles (PubMed:16541025). Centromeric localization requires the presence of SGO2 (By similarity). .
• 信号通路 Oocyte meiosis;WNT;WNT-T CELLTGF-beta;Tight junction;Long-term depression;
• 功能 catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,function:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.,PTM:Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.,PTM:Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.,similarity:Belongs to the PPP phosphatase family.,similarity:Belongs to the PPP phosphatase family. PP-1 subfamily.,subcellular location:In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.,subunit:PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct (By similarity). May indirectly interact with SGOL1, most probably through regulatory B56 subunits.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of A549 cells, using PP2A-alpha （Phospho-Tyr307） Antibody. The picture on the right is blocked with the phospho peptide.
	Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha （Phospho-Tyr307） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from A549 cells, using PP2A-alpha （Phospho-Tyr307） Antibody. The lane on the right is blocked with the phospho peptide.
	Western blot analysis of SKOV3 using p-PP2A-Cα （Y307） antibody. Antibody was diluted at 1:1000
	Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
	Immunofluorescence analysis of Hela cell. 1,PP2A-Cα (phospho Tyr307) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). HSP70 Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

产品详情