产品基本信息

• 产品货号 BD-PP0815
• 别名 CTBP1; CTBP; C-terminal-binding protein 1; CtBP1
• 产品名称 CtBP1 (phospho Ser422) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CTBP1
• 蛋白名称 C-terminal-binding protein 1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1487
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1487
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q13363/entry
• Mouse Gene ID 13016
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13016
• Mouse Swissprot No. O88712
• Mouse Swissprot Link http://www.uniprot.org/uniprot/O88712
• Rat Gene ID 29382
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29382
• Rat Swissprot No. Q9Z2F5
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q9Z2F5
• 免疫原 The antiserum was produced against synthesized peptide derived from human CtBP1 around the phosphorylation site of Ser422. AA range:388-437
• 特异性 Phospho-CtBP1 (S422) Polyclonal Antibody detects endogenous levels of CtBP1 protein only when phosphorylated at S422.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 48kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008],
• 组织表达 Expressed in germinal center B-cells.
• 细胞定位 Cytoplasm . Nucleus .
• 信号通路 WNT;WNT-T CELLNotch;Pathways in cancer;Chronic myeloid leukemia;
• 功能 cofactor:NAD. Required for efficient interaction with E1A. Cofactor binding induces a conformation change.,function:Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex (By similarity). Co-repressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.,PTM:ADP-ribosylated; when cells are exposed to brefeldin-A (BFA).,PTM:Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.,PTM:The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.,similarity:Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.,subunit:Interacts with the C-terminus of adenovirus E1A protein, ELK3 and CTIP via their consensus motif P-X-[DNS]-L-[STVA]. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with FOXP2, HDAC4, HDAC5 and HDAC9. Interacts with GLIS2 but not GLIS1 or GLIS3 (By similarity). Interacts with FOXP1, HIPK2, PNN and NRIP1. Interacts with ZFHX1B and WIZ. Interacts with Epstein-Barr virus EBNA3 and EBNA6.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human brain, using CtBP1 （Phospho-Ser422） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30‘ and Jurkat cells treated with UV 15‘, using CtBP1 （Phospho-Ser422） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of JK cells using Phospho-CtBP1 （S422） Polyclonal Antibody

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