产品基本信息

• 产品货号 BD-PP0753
• 别名 IRAK1; IRAK; Interleukin-1 receptor-associated kinase 1; IRAK-1
• 产品名称 IRAK-1 (phospho Thr100) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 IRAK1
• 蛋白名称 Interleukin-1 receptor-associated kinase 1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 3654
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3654
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P51617/entry
• Mouse Gene ID 16179
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16179
• Mouse Swissprot No. Q62406
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q62406
• 免疫原 The antiserum was produced against synthesized peptide derived from human IRAK1 around the phosphorylation site of Thr100. AA range:66-115
• 特异性 Phospho-IRAK-1 (T100) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at T100.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 77kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
• 组织表达 Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
• 细胞定位 Cytoplasm . Nucleus . Lipid droplet . Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity). .
• 信号通路 Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Toll_Like;Neurotrophin;
• 功能 catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.,PTM:Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.,similarity:Contains 1 protein kinase domain.,subunit:IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Interacts with IL1RL1. Interacts with IRAK1BP1.,tissue specificity:Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using IRAK1 （Phospho-Thr100） Antibody
	Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRAK1 （Phospho-Thr100） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from Jurkat cells treated with heat shock, using IRAK1 （Phospho-Thr100） Antibody. The lane on the right is blocked with the phospho peptide.

产品详情