产品基本信息

• 产品货号 BD-PP0745
• 别名 SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
• 产品名称 Smad3 (phospho Thr179) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 SMAD3
• 蛋白名称 Mothers against decapentaplegic homolog 3
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 4088
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4088
• Human Swissprot No. P84022
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P84022/entry
• Mouse Gene ID 17127
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17127
• Mouse Swissprot No. Q8BUN5
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q8BUN5
• Rat Gene ID 25631
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25631
• Rat Swissprot No. P84025
• Rat Swissprot Link http://www.uniprot.org/uniprot/P84025
• 免疫原 The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Thr179. AA range:145-194
• 特异性 Phospho-Smad3 (T179) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at T179.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 50kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],
• 组织表达 Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood
• 细胞定位 Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .
• 信号通路 Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;WNT;WNT-T CELLTGF-beta;Adherens_Junction;Pathways in cancer;Colorectal cancer;Pancreatic cancer;Chronic myeloid leukemia;
• 功能 disease:Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].,domain:The MH2 domain is sufficient to carry protein nuclear export.,function:Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with Smad4.,subunit:Interacts with HGS. Interacts with NEDD4L in response to TGF-beta. Interacts with TTRAP (By similarity). Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4. Interacts with JUN/FOS, vitamin D receptor, homeobox protein TGIF and TGIF2, PEBP2-alpha C subunit, CREB-binding protein (CBP), p300, SKI, SNON, ATF2, SMURF2, AIP1, DACH1 and TGFB1I1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Found in a complex with SMAD2 and TRIM33 upon addition of TGF-beta. Interacts with SMAD2 and TRIM33. Found in a complex with SMAD3, Ran and XPO4. Interacts with XPO4. Interacts with LBXCOR1 and CORL2.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human heart, using Smad3 （Phospho-Thr179） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 2‘, using Smad3 （Phospho-Thr179） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of HELA cells using Phospho-Smad3 （T179） Polyclonal Antibody

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