产品基本信息

• 产品货号 BD-PP0730
• 别名 CDC25B; CDC25HU2; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B
• 产品名称 Cdc25B (phospho Ser353) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CDC25B
• 蛋白名称 M-phase inducer phosphatase 2
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Monkey
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 994
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=994
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P30305/entry
• Mouse Swissprot No. P30306
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P30306
• 免疫原 The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser353. AA range:319-368
• 特异性 Phospho-Cdc25B (S353) Polyclonal Antibody detects endogenous levels of Cdc25B protein only when phosphorylated at S353.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 64kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 cell division cycle 25B(CDC25B) Homo sapiens CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008],
• 组织表达 Brain,Rectum tumor,
• 细胞定位 Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .
• 信号通路 MAPK_ERK_Growth;MAPK_G_Protein;Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Progesterone-mediated oocyte maturation;
• 功能 catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,enzyme regulation:Stimulated by B-type cyclins.,function:Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.,PTM:Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using CDC25B （Phospho-Ser353） Antibody
	Immunohistochemistry analysis of paraffin-embedded human brain, using CDC25B （Phospho-Ser353） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from COS7 cells treated with etoposide 25uM 24h, using CDC25B （Phospho-Ser353） Antibody. The lane on the right is blocked with the phospho peptide.

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