产品基本信息

• 产品货号 BD-PP0720
• 别名 PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
• 产品名称 PKD1 (phospho Ser205) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 PRKD1
• 蛋白名称 Serine/threonine-protein kinase D1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 5587
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5587
• Human Swissprot No. Q15139
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q15139/entry
• Mouse Gene ID 18760
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18760
• Mouse Swissprot No. Q62101
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q62101
• Rat Gene ID 85421
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=85421
• Rat Swissprot No. Q9WTQ1
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q9WTQ1
• 免疫原 The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu around the phosphorylation site of Ser205. AA range:171-220
• 特异性 Phospho-PKD1 (S205) Polyclonal Antibody detects endogenous levels of PKD1 protein only when phosphorylated at S205.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:40000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 110kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010],
• 组织表达 Placenta,Testis,
• 细胞定位 Cytoplasm . Cell membrane . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation.
• 信号通路 Regulation_Microtubule; Regulation of Actin Dynamics; Stem cell pathway; Insulin Receptor; B Cell Receptor; AMPK
• 功能 catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase involved in resistance to oxidative stress.,PTM:Phosphorylation of Ser-738 and/or Ser-742 in activated PKD is mediated by transphosphorylation (By similarity). Phosphorylation of Tyr-463 mediated by the Src/Abl pathway in response to oxidative stress activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts (via N-terminus) with ADAP1/CENTA1. Interacts with Src.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using PKD1/PKC mu （Phospho-Ser205） Antibody
	Immunohistochemistry analysis of paraffin-embedded human brain, using PKD1/PKC mu （Phospho-Ser205） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from NIH/3T3 cells treated with Anisomycin 25ug/ml 30‘, using PKD1/PKC mu （Phospho-Ser205） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of 3T3 cells using Phospho-PKD1 （S205） Polyclonal Antibody

产品详情