纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
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1ug Flag fusion protein+ Primary antibody dilution at 1) 1:5000;2) 1:20000 Transfected 293 cell lysate with Flag-tag fusion protein+ Primary antibody dilution at 1:20000. Secondary antibody was diluted at 1:20000