产品基本信息

• 产品货号 BD-PP0664
• 别名 RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas
• 产品名称 p70 S6 kinase α (phospho Ser371) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 RPS6KB1 STK14A P70S6K
• 蛋白名称 Ribosomal protein S6 kinase beta-1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 6198
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6198
• Human Swissprot No. P23443
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P23443/entry
• Mouse Gene ID 72508
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=72508
• Mouse Swissprot No. Q8BSK8
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q8BSK8
• Rat Gene ID 83840
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=83840
• Rat Swissprot No. P67999
• Rat Swissprot Link http://www.uniprot.org/uniprot/P67999
• 免疫原 The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Ser371. AA range:337-386
• 特异性 Phospho-p70 S6 kinase α (S371) Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein only when phosphorylated at S371.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 60kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],
• 组织表达 Widely expressed.
• 细胞定位 Cell junction, synapse, synaptosome . Mitochondrion outer membrane. Mitochondrion. Colocalizes with URI1 at mitochondrion.; [Isoform Alpha I]: Nucleus. Cytoplasm.; [Isoform Alpha II]: Cytoplasm.
• 信号通路 Regulates Angiogenesis; Insulin Receptor; ErbB/HER; mTOR; B Cell Receptor; PI3K/Akt; PI3K/Akt; AMPK
• 功能 catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activation by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase.,function:Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PPP1R9A/neurabin-1.,tissue specificity:Widely expressed.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using p70 S6 Kinase （Phospho-Ser371） Antibody
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase （Phospho-Ser371） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from COLO205 cells treated with serum 20% 15‘, using p70 S6 Kinase （Phospho-Ser371） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of COLO cells using Phospho-p70 S6 kinase α （S371） Polyclonal Antibody diluted at 1：500

产品详情